ZFIN Image: Tang et al., 2016, Fig. S4

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Figure Caption

Fig. S4

Visualising neuroblastoma and ERMS metastasis in transplanted rag2^E450fs (casper) fish.

(a) Retro-orbital transplantation of a GFP-labelled neuroblastoma. White arrow indicates the site of injection; yellow arrow indicates metastasis to a region near the liver. (b) Intra-muscular transplantation of ERMS into rag2^E450fs recipient fish. White arrow indicates the site of injection; yellow arrows indicate the site of metastasis. Note that metastasis can be directly visualised by epifluorescence microscopy in the rag2^E450fs (casper) recipients (yellow arrow) but not the rag2^E450fs (AB) (open yellow arrow). H&E and anti-GFP staining on sections of the recipient animals confirmed the location of metastasis. Scale bars equal 5 mm for whole animal images, 2 mm in images of heads, 1 mm in 40x histological images; 300 µm in 100x histological images; and 100 µm in 400x histological images.

Acknowledgments

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