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Fig. 1
Isolation of LSD1 mutant. (A) Expression of gata1 in WT sibling (WT-sib) and it627 embryos. The arrows indicate hematopoietic tissues: posterior LPM (14 hpf) and ICM (22 hpf). (B) Structures of the LSD1 proteins. SWIRM, Swi3p, Rsc8p, and Moira domain; Tower, tower domain. (C) Results of the demethylation assay using bacterially expressed LSD1 proteins and bulk histones from calf thymus as substrates. Methylated proteins were detected by immunoblotting using specific antibodies.
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