Fig. 4 Decreased expression of lymphoid markers in gene-trap mutant embryos.
(A-F) Whole mount RNA in situ hybridization (WISH) of lck in 5 dpf larvae from the lines fcc143 (143) (A-B), agtpbp1fcc301 (301) (C-D) and eps15L1fcc436-P1 (436) (E-F). Representative embryos displaying no GFP (A, E) or normal expression of lck (C) are compared to siblings with strong (str) GFP (B, F) or low levels of lck expression (D). In line agtpbp1fcc301, the GFP expression levels varied, making it difficult to distinguish heterozygous and homozygous carriers. The number of embryos that showed the representative phenotype is indicated in panels C and D.