ZFIN Image: Xue et al., 2015, Fig. S2

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Figure Caption

Fig. S2 Expression of klf3 and klf6a during zebrafish embryogenesis.

(A) Expression of klf3 from 1-cell stage to 36 hpf. klf3 is ubiquitously expressed before 1K cell stage at low level, but after bud stage, is expressed in PLM at 10 somite stage, then in ICM region at 24 hpf. At 36 hpf, expression of klf3 is detected in hatching gland and pronephric duct. The black arrowheads indicate the specific expression. (B) Expression of klf6a from 1-cell stage to 36 hpf. klf6a expression begins from 1-cell and then is observed in neural plate and notochord at 10 somite stage. klf6a is expressed in dorsal neurons and blood vessels including head and caudal region at 24 hpf and 36 hpf. The expression of klf6a is marked by arrowheads. (C) The expression of klf3 and klf6a was determined by qPCR using gata1 positive cells sorted from flk1:GFP/gata1:dsRed transgenic embryos by flow cytometry. gata1 expression was used as positive control and flk1 as negative control.

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 403(2), Xue, Y., Gao, S., Liu, F., Genome-wide Analysis of the Zebrafish Klf Family Identifies Two Genes important for Erythroid Maturation, 115-27, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.