Fig. S3 nrg2a mutant larvae have altered pectoral fin folds, lack swim bladders and die between 8 and 12 dpf.
(A-D) Images of live larvae at 7 dpf. (A) Wild-type pectoral fin folds (PFF) typically follow a continuous arc such that the PFF edge lies close to the body when larvae are at rest. (B) PFFs in nrg2a mutant larvae (mn0237Gt/mn0237Gt) often depart from a continuous arc shape and/or have thickened edges similar to the MFF phenotype (arrowhead). In addition, wild-type larvae have well-developed swim bladders (C), but swim bladders fail to develop in mn0237Gt/mn0237Gt mutant larvae (D, arrowhead). (E) Graphical illustration of survival rates of mn0237Gt/mn0237Gt mutant larvae (n = 104 larvae per class).