Fig. 5

Fig. 5

klf6a knockdown induces a blockage of erythroid cell differentiation and maturation. (A) WISH results showed that gata1 expression was comparable between controls and klf6a morphants from 24 hpf. The expression of be1-globin and be3-globin was decreased in klf6a morphants, compared with controls at 36 hpf and 2 dpf. (B) The number of o-dianisidine-positive cells (marked by arrowheads) decreased dramatically in klf6a morphants between 40 hpf and 2 dpf. (C) The real time PCR results displayed that ae1-globin and be1-globin were downregulated in klf6a morphants at 36 hpf and 2 dpf (mean±SD, *P <0.05, **P <0.01, n=3). (D) Giemsa staining of erythroid cells from the blood of controls and klf6a morphants at 2.5 dpf. The size and nucleo-cytoplasmic ratio of red blood cells in klf6a morphants were noticeably larger than those of controls. The arrowhead marks the delayed erythroid progenitor. (E) Quantification of the nucleo-cytoplasmic ratio between controls and klf6a morphants (mean±SD, ^NNP <0.01, n=19).