ZFIN Image: Wong et al., 2015, Fig. 3

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Fig. 3

Expression pattern of dmbx1a in embryos with varying vsx2 levels. In situ hybridization of dmbx1a gene expression at 48 hpf in uninjected embryos (A), induced pSGH:vsx2 injected embryos (B) and vsx2 morphants (C). RT-qPCR was used to quantify RNA from three pools of 50 embryos collected from uninjected, pSGH:vsx2 injected, and vsx2 morpholino injected embryos (D and E). There was a significant decrease in dmbx1a expression at 72 hpf when vsx2 is over expressed. In contrast, there was a significant increase at 48 hpf in dmbx1a expression when vsx2 was knocked down. Standard deviation was calculated from three replicate experiments. Statistical significance was accomplished using Student′s t-test (p<0.05).

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Acknowledgments

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Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.