|
Fig. 10 Pharmacological inhibition of PrkC activity reduces apical domain size. (A–C) Transverse cryosections of 4 dpf retinas were stained with phalloidin to label actin (red) and zpr-1 to label double cones (green). (A) DMSO-control treated larvae; (B) Larvae treated with 3 μM PrkC inhibitor; (C) Larvae treated with 3 μM control inhibitor. Sections were also counterstained with DAPI (blue). (D) Quantification of apical domain as a percentage of total photoreceptor inner segment length. All values are plotted as a percentage of n/m, as defined in the text. Control = 45%; PrkC inhibitor = 28%; non-myristoylated control inhibitor = 44%. Blue error bars indicate the mean ± standard deviation. Scale bar = 10 μm.