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Fig. S3 p-S6K signals were markedly reduced by 3 or 6 h treatment with inhibitors (rapamycin, Torin1, and AZD8055). (A) Scheme of rapamycin, Torin1, or AZD8055 treatment from 24 h to 27 or 30 hpa. (B-I) Longitudinal sections of wild-type fin regenerates that were immunohistochemically stained with an antibody against p-S6K (green) at 27 hpa (DMSO, n = 3; Rapa, n = 3; Torin1, n = 3; AZD8055, n = 3) or 30 hpa (DMSO, n = 3; Rapa, n = 3; Torin1, n = 3; AZD8055, n = 3). DAPI fluorescent signal (blue) indicates the presence of nuclei. The p-S6K signals were markedly reduced by 3 or 6 h treatment with inhibitors (rapamycin, Torin1, and AZD8055). Dashed white lines indicate the amputation plane. Scale bars: 100 µm.