ZFIN Image: Li et al., 2014, Fig. 6

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Fig. 6

RA negatively regulates mecom, which enables MCC formation. (A) Wild type embryos treated with 1×10⁷ M RA, 1.6×10⁵ M DEAB or DMSO. Analysis of mecom transcripts using WISH shows that RA treatment diminishes the mecom domain while abrogation of RA signaling expands the mecom domain. (B) MCCs were labeled by odf3b in wild types and mecom morphants treated with RA or DEAB. mecom knockdown resulted in a caudal shift of MCC domain in the DL and PD regions, and this effect was partially rescued by treating morphant embryos with DEAB. Notably, wild type embryos treated with DEAB had abolished MCC formation, while RA induced ectopic MCC formation in the distal region of the pronephros.

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Acknowledgments

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Reprinted from Developmental Biology, 386(1), Li, Y., Cheng, C.N., Verdun, V.A., and Wingert, R.A., Zebrafish nephrogenesis is regulated by interactions between retinoic acid, mecom, and Notch signaling, 111-122, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.