ZFIN Image: Maier et al., 2014, Fig. 9

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Figure Caption

Fig. 9

RA signalling and FGF signalling are linked in a regulatory feedback loop in the ear.

Embryos were treated with DMSO, SU5402 and DEAB from 18S to 26 hpf, with RA from 20S to 26 hpf, and heatshock was performed at the 18/20S stage. (A–H) aldh1a3 expression is normal in sibling embryos (n = 24/31 embryos from a heterozygous cross (ace sib), n = 31/39 (lia sib)) but lost in embryos mutant for fgf8a (ace, n = 7/31; C,D) or fgf3 (lia, n = 8/39; G,H). (I–L) aldh1a3 expression is normal in WT embryos treated with DMSO (n = 15; I,J), but lost in embryos treated with 10 µM SU5402 (n = 35; K,L). (M–P) Tg(hsp70:fgf3) embryos were heat shocked (HS) from 18S and sorted as non-green non-transgenic siblings (M,N), or green transgenic embryos (O,P), and double stained for aldh1a3 (blue) and otx1b (red). In non-green non-transgenic embryos, expression of aldh1a3 is normal (n = 31). In green Tg(hsp70:fgf3) embryos, expression of aldh1a3 and otx1b is up-regulated (n = 48). (Q–S) fgf3 expression is normal in WT embryos treated with DMSO (n = 15; Q) but down-regulated in the otic epithelium in embryos treated with 10 nM RA (n = 28; R); the domain is expanded in embryos treated with DEAB (n = 23; S). (T–V) fgf8a expression is normal in WT embryos treated with DMSO (n = 16; T) but down-regulated in the otic epithelium in embryos treated with 10 nM RA (n = 22; U) and expanded posteroventrally in embryos treated with DEAB (n = 19; V). (W–Y) dusp6 expression is normal in WT embryos treated with DMSO (n = 9; W) but down-regulated in the otic epithelium in embryos treated with 10 nM RA (n = 26; X) and expanded posteroventrally in embryos treated with DEAB (n = 17; Y). (Z–Z3) Tg(hsp70:dnRAR) embryos were heat shocked (HS) from 18S and sorted as non-green non-transgenic siblings (Z) or green transgenic embryos (Z′,Z′′). In non-green, non-transgenic embryos, the expression of fgf3 is normal (n = 18; Z). In green and very green Tg(hsp70:dnRAR) embryos the expression fgf3 is up-regulated (n = 28; Z′,Z′′). Dotted line demarcates the OV. Scale bar: 50 µm.

Figure Data

Expression / Labeling details

Acknowledgments

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