ZFIN Image: Casari et al., 2014, Fig. S11

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Figure Caption

Fig. S11 In vivo blocking of Smad3/TGFβ signaling impairs neuronal differentiation during early larval development. Confocal Z-stack images of the trunk of 72 hpf transgenic embryos expressing GFP under control of either a glial or neuronal promoter: ngn1, neuroD and mnx1. All these transgenic lines have been treated at 24 hpf with Alk5-inhibitor LY364947 and confocal observation performed after two days of treatment. Disturbing Smad3/TGFβ signaling at early larval development causes an increase of proliferative cells (ngn1), decrease of differentiating cells (neuroD) and reduction of late differentiating motor neurons (mnx1).

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Reprinted from Developmental Biology, 396(1), Casari, A., Schiavone, M., Facchinello, N., Vettori, A., Meyer, D., Tiso, N., Moro, E., Argenton, F., A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development, 81-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.