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Fig. 4 Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: ACAACATCCAGTGGCTAGGG. Reverse: AATTAACCCTCACTAAAGGGACGGAGACAGGCAGATAAAGC. PCR product size: 653 bp. Not spatially restricted.
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