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Fig. 4
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Fig. 4
Discrete cholinergic and peptidergic subnuclei of adult dHb. Transverse sections through the Hb of adult brains processed for vactb (A), chatb (B), and hacta (C) expression. (D) Double fluorescent ISH for vachtb and tac1 confirm nonoverlapping cholinergic (green) and tac1-positive (magenta) neuronal populations in the left dHb. Composite z-stack images taken from coronal sections of left (E) and right (F) Hb. Coexpression of vachtb (magenta) and Tg(brn3a-hsp70:GFP) (green). (D–F) White dashed lines delineate dHb, and gray dotted lines delineate vHb. (Scale bars: 50 &mum.)
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