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Fig. 8
Def haploinsufficiency caused an altered migration behaviour of leukocytes in def+/- fish that was rescued by treatment with Dex or ectopic expression of Def in hepatocytes.
(A–H) Representative images of co-immunostaining of EGFP (green, label macrophage), dsRed (yellow, label neutrophil) and Bhmt (red) in the wound epidermis in wild-type (A,B), def+/- (C,D), def+/- treated with Dex (E,F) and def+/-Tg(fabp10a:def) progeny (G,H) 12 and 36 h after PH, respectively. All are in the background of Tg(coro1a:eGFP;lyz:dsRED). Nuclei were stained with DAPI (blue). In each case, more than 10 sections from at least three wild-type or def+/- mutant fish were examined. bc, blood clot; lv, liver tissue. Scale bars: 150 μm (A–H).