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Fig. 1
def+/- liver suffers from haploinsufficiency of Def.
(A) qPCR analysis of def transcripts in adult wild-type and def+/- liver. The expression of def was normalised against elf1a and is shown as the fold change in expression, with the value from def+/- being set at 1. The values plotted represent the means ± standard errors of the mean. (B) Western blotting analysis of the Def protein. Bhmt: loading control. Densitometry was performed using Photoshop. The value of Def was obtained by normalising against Bhmt expression. The relative value of Def protein in def+/- was set at 1. (C) Representative images of immunostaining of Def (red) in wild-type and def+/- liver 2 days after PH. lv, liver tissue. Scale bar: 100 μm (C).