Fig. 3
Defects in neuromast formation were observed in both Rtn4-m/Nogo-C2 and NgRH1a morphants.
Zebrafish embryos were injected with morpholinos against the indicated zebrafish Nogo-related ligand or Nogo receptor at the 2~4-cell stage. Neuromast formation in these morphants was observed by staining with 4-Di-2-ASP at 72 h post-fertilization (hpf). A control embryo is shown in panel (A). Images were taken from the lateral view, and the yellow dots indicate neuromasts of the zebrafish lateral line. Scale bar, 100 μm. A summary of PLL neuromast numbers in MO-injected embryo is shown in panel (I). Sample size (N) and MO dosage used are indicated.