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Fig. 6
Absence of cad function in homozygous mutant embryos leads to proliferation defects and massive cell death in the midbrain. (A,B) Sagittal sections of wild-type (A) and perplexed (B) embryos with Nissl staining at 48 hpf. perplexed mutant displays atrophy of the tectum and PML thickening. (C,D) Sagittal sections following TUNEL staining at 48 hpf. More apoptotic cells are observed in cad-/- (D) than in wild-type (C) embryos. (E,F) Phospho-histone H3 labelling at 48 hpf showing the presence of proliferative cells not only in the periphery of the tectum but also in the central part in perplexed mutants. (G-J) Pcna immunostaining at 72 and 96 hpf showing persistence of wide proliferation zones in the OT of perplexed mutants at late larva stages. Anterior is at the left and dorsal at the top of each image. OT, optic tectum, Ce, cerebellum. The asterisk indicates the PML. Scale bars: 100 μm in A,B; 10 μm in C,D; 20 μm in G; 50 μm in E,F,H-J.