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Fig. 8 pax8 acts downstream of foxi1 and in parallel with dlx3b. Wild-type embryos injected with foxi1-MO (A-C), foxi1-MO plus pax8-MO (D-F), dlx3b-MO (G-I), or dlx3b-MO plus pax8-MO (J-L). Images show lateral views of the otic vesicle at 30 hpf (A,D,G,J), or dorsal views of dlx3b expression at 12 hpf (B,E,H,K) and pax2a expression at 12 hpf (C,F,I,L). Anterior is to the left in all specimens. mhb, midbrain-hindbrain border; op, otic placode. Arrowheads mark the otic region of dlx3b expression. Scale bar in L: 50 μm for A,D,G,J; 200 μm for B,C,E,F,H,I,K,L.
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