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Fig. 2 Assessment of Pax8 function in otic induction. (A,D) Head region of live wild-type (A) and pax8-MO injected (D) embryos at 30 hpf. The pax8-MO-injected embryo has a narrow midbrain-hindbrain border (asterisk), and small otic vesicles (ov). (B,E) Otic vesicles at 30 hpf in live wild-type (B) and pax8-MO-injected (E) embryos. (C,F) Otic vesicles at 30 hpf in embryos injected with v1-MO to knockdown variant 1 isoforms of pax8 (C) or v2/3-MO to knockdown variant 2 and 3 isoforms of pax8 (F). (G,J) pax8 expression at 10 hpf in wild-type (G) and pax8-MO-injected (J) embryos. (H,K) Expression of pax2a (blue) and krox20 (red) at 12 hpf in wild-type (H) and pax8-MO-injected (K) embryos. (I,L) dlx3b expression at 12 hpf in wild-type (I) and pax8-MO-injected (L) embryos. Images show lateral views with anterior to the left and dorsal to the top (A-F); or dorsal views with anterior to the left (G-L). mhb, midbrain-hindbrain border; op, otic placode; ov, otic vesicle. Scale bar in L: 200 μm for H,K; 170 μm for G,I,J,L; 150 μm for A,D; 40 μm for B,C,E,F.