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Fig. S9
Simultaneous knockdown of sox2 and BMP rescues the number of PhRs. (A) DMSO-treated control embryos at 28 hpf, with PhR in green (Tg(aanat2:GFP)) and Sox2 in red. (B) Dorsomorphin treatment, that inhibits the BMP pathway, leads to a reduced number of PhRs, while Sox2 expression is normal. (C) Knockdown of sox2 leads to increased number of PhRs. (D) Simultaneous inhibition of sox2 and BMP results in a number of PhR similar to the DMSO-treated control siblings. (E) Average number of PhR in DMSO-treated (purple bar), dorsomorphin-treated (green bar), sox2 morphant treated with DMSO (orange bar) and sox2 morphant treated with dorsomorphin (red bar) embryos. Confocal maximum projections of 28 hpf embryos, error bars represent ± standard error, * = p-value <0.05 (MWU test), ** = p-value <0.001 (MWU test).