Fig. S8

Fig. S8

Downregulation of BMP does not affect sox2 expression at 15 ss. (A-E′′) sox2 is expressed throughout the pineal anlage and is downregulated in isl1-positive cells, in DMSO-treated control embryos. (A-E) sox2 expression, (A′-E′) isl1 expression, (A′′-E′′) merged images of sox2, isl1 and Tg(flh:GFP), showing the presumptive pineal gland. (F-J′′) Dorsomorphin treatment (40 µM) does not affect sox2 expression at 15 ss. sox2 is expressed in the pineal precursors and is downregulated with differentiation (as shown by isl1-positive cells). (F-J) sox2 expression, (F′-J′) isl1 expression, (F′′-J′′) merged images of sox2, isl1 and Tg(flh:GFP). Series of optical sections from dorsal (first column) to ventral (5^th column) obtained using confocal microscope, scale bars = 25 µm, yellow arrows show cells in which sox2 is downregulated and isl1 is upregulated, insets show a three times magnified view of the image. See also Figure 6, Movie S3.