Fig. 5

Fig. 5

CHIA expression patterns at pharyngula through to the early larval stages. (A–C) 2 DPF, (D–F) 3 DPF. The bar represents 1 mm except in F where it represents 0.4 mm. (A) 2 DPF panel of all CHIA (1–6) gene expression patterns. The lens of the eye and the caudal aorta seem to be the major tissues of expression of all the CHIA genes. The pectoral fin buds are visible in dorsal views of CHIA.2, CHIA.5 and CHIA.6 (arrowheads in A2, A5 and A6). (B) 2 DPF lateral close-up. The primary head sinus (PHS) can be seen as a curved line along the anteroposterior axis posterior to the eye. Extending dorsally immediately posterior to the tectum runs what appears to be the middle cerebral vein (MCV). Along the tail the caudal aorta (CA) can be seen extending all along the trunk and tail. (C) 2 DPF dorsal close-up. The lens (L) displays a strong signal at this stage. (D) 3 DPF panel of all CHIA gene expression patterns. (E) 3 DPF lateral close-up. The head blood vessels (HBV) can be seen at several places in the brain region. The tissue around the newly opened mouth (M), and the pharynx (P) and pharyngeal arches (PA) can be distinguished. The heart (H), caudal aorta (CA) and intestinal bulb (IB) also exhibit clear expression. (F) Dorsal close-up of the eye: the inner plexiform (IP) layer forms a clear cup shaped symmetrical pattern around the lens. Faint expression is visible in the optic nerve (ON) extending from the lens.