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Fig. 5 fz activity does not require putative PDZ binding motif. (A) Representation of fzAΔPBM, a fzA protein lacking the putative PDZ binding motif (see also Fig. 1A). Panels B-D and F-G show effects of fzΔPBM misexpression. (B) 28 hour embryo, showing dorsalized phenotype. Note reduced trunk and tail structures. (C, D) fzΔPBM induced axis duplication. Primary (1°) and secondary (2°) axes are indicated. y designates yolk. GFP is expressed mainly in the ectopic head (D). Expansion of (F), and ectopic (G) dorsal organizer tissue in fzΔPBM-injected embryos, as revealed by gsc expression in these 65% epiboly embryos