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Fig. 7 Pax2.2 expression is independent of the noi (Pax2.1) function. Transcripts of the Pax2.2 gene were analysed by whole-mount in situ hybridization in wild-type (A,C) and noitu29a mutant (B,D) embryos at 7 somites and 20 hours postfertilization. The 7-somite embryos (A,B; lateral view) were genotyped by double staining with Pax2.2 (blue) and eng3 (red) RNA probes. Homozygous noi mutant embryos were identified by the absence of engrailed (eng) expression at the midbrain-hindbrain boundary (mhb) (Brand et al., 1996). At 20 hours, the mhb tissue is entirely lost in noi mutant embryos (dorsal view).