ZFIN Image: Akitake et al., 2011, Fig. 4

Image

Figure Caption

Fig. 4 Correlation between variegated expression and CpG methylation. (A) In the c368 line, variegation in GFP labeling was observed in F1 larvae (not shown) and was more frequently observed in F2 and F3 larvae compared to other 4Xnr UAS lines (compare with Fig. 3B). Few c368 GFP^high larvae were found in either generation. (B) Fluorescence images and representative DNA bisulfite sequencing data for individual GFP^med and GFP^low c368 F2 larvae at 2 dpf. The upper larvae possess both the c368a and c368b transgenic insertions (Table 2), whereas the bottom two larvae have only the c368a transgene. GFP-labeled individuals carrying only the c368b insertion were not detected.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 352(2), Akitake, C.M., Macurak, M., Halpern, M.E., and Goll, M.G., Transgenerational analysis of transcriptional silencing in zebrafish, 191-201, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.