ZFIN Image: Rothschild et al., 2013, Fig. 2

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Figure Caption

Fig. 2 Otolith formation is defective in camk2g1 morphants. Gross morphology of otoliths was assessed at 30 hpf (A–C) and 72 hpf (D–F) in embryos injected with 1 ng control MO (A, D) or 1 ng camk2g1 MO (B, C, E, F). Scale bar (50 μm) in A applies to A–C and in D applies to D–F. Defects in otolith shape were quantitated at 72 hpf for 186 morphant embryos and compared to 118 control embryos (G). P-CaMK-II (red) at the base of the kinocilia in 24 hpf control (H, H′) embryos (arrowhead) was lost in camk2g1 morphants (I, I′). Cilia were counterstained with anti-acetylated tubulin (green) with anterior on the left. Scale bars in H, I=5 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 381(1), Rothschild, S.C., Lahvic, J., Francescatto, L., McLeod, J.J., Burgess, S.M., and Tombes, R.M., CaMK-II activation is essential for zebrafish inner ear development and acts through Delta-Notch signaling, 179-88, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.