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Fig. 2
Treatment with beclomethasone caused impaired cardiac repair in zebrafish.
Objects were sacrificed at 30 days post ventricular resection; heart sections were stained with aniline blue to distinguish scar tissue from normal tissue. Blue: scar tissue; Orange: normal tissue. (A, A′) In the vehicle control group, the zebrafish regenerated the injured hearts perfectly 1 month after injury. Only a few scar tissue (arrow) was detected in the wound (n = 17). (B, B′) Exposure to beclomethasone (0.25 μM) hindered the cardiac repair process. A large amount of scar tissue (arrow) was found in the wound instead of renewed cardiomyocytes. Meanwhile, obvious blood clots remained, which could be detected (arrow head) (n = 14, scale bar = 100 μm). The dashed lines indicate the approximate amputation plane.