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Fig. 2
hoxd4a knockdown disrupts primitive hematopoiesis and is highly specific.
In situ hybridization revealing expression at 13 hpf of erythroid lineage markers gata1 (A,C,E) and β embryonic globin 1 (hbbe1) (B,D,F). (A,B) Normal expression of gata1 and hbbe1 in the LPM. (C,D) Expression of gata1 and hbbe1 is strongly down-regulated in hoxd4a morphants, but rescued by co-injection of capped mRNA for hoxd4a (E,F). All embryos have been flat-mounted and are shown in dorsal view. Anterior is to the left. Ratios in the bottom left corner of all panels indicate the number of embryos showing the presented phenotype. ctrl, embryos injected with a non-specific morpholino. MO, embryos injected with the anti-hoxd4a morpholino. hoxd4a mRNA, embryos simultaneously injected with the anti-hoxd4a MO plus capped mRNA for hoxd4a. Scale bars equal 100 µm. All images are at the same magnification.