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Fig. S3 (A and B) Single-neuron labeling through plasmid injection in a cldnb-gfp embryo. (A and A′) At 5 days post fertilization (dpf), the neuron innervates a single neuromast. (B and B′) Same fish 1 mo later. The founder neuromast is flanked by a fully differentiated bud neuromast, and both neuromasts are generating a new bud on either side. In both cases, neurites (arrows) invade the bud structures (arrowheads) well before the bud neuromasts begin to differentiate. (C and D) Expression of lgr6 in neuromast cells at 3 dpf (C) and at 11 dpf (D). [Scale bars: 20 μm (C) and 10 μm (D).] (E and F) Rspo2 enhancer trap activity in the neurites that innervate ter1 and ter2 at 7 dpf (E) and in a branch innervating bud neuromast ter2′ at 11 dpf (F). (F) Composite of two frames from a confocal stack, 5.2 μm apart. Maximum projection cannot be used because of high reporter activity in the surrounding mesenchyme.