ZFIN Image: Ma et al., 2012, Fig. 1

Image

Figure Caption

Fig. 1 T-cell development is impaired in zebrafish foxn1 morphants. (A and B) The endogenous expression level of foxn1 transcript and the encoded protein in zebrafish foxn1 morphants at 4 dpf detected by WISH (A) and Western blot (B). Ba, Western blot; Bb, Western blot results were quantified by using Quantity 1 software. (C) The rag2:dsRed expression was abolished in foxn1 morphants at 5 dpf. (D) The expression of lymphocyte markers, including rag1, il7r, and ikaros, was down-regulated in the thymus in zebrafish foxn1 morphants. Anterior to the left and dorsal to the top; circles mark the thymus. (E) qRT-PCR results showing that the expression of rag1, il7r, and ikaros is down-regulated in zebrafish foxn1 morphants (mean ± SD, t test, **P < 0.01, n = 3).

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA