ZFIN Image: Wu et al., 2012, Fig. 5

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Figure Caption

Fig. 5

Inhibition of Ccr7 and intracellular calcium promotes expression of dorsal genes.

(A) Expression of β-catenin downstream targets, boz, mkp3, and sqt, in ich mutants (a–d) and MO1-ccr7-injected ich mutants (a2–d2) during early blastula stages revealed by WISH. (a–a2) 3.3 hpf, boz, n = 11/14; (b–b2) boz, n = 6/11; and 4 hpf (c–c2) mkp3, n = 8/8; (d–d2) sqt, n = 8/8. Animal views, dorsal to the right. (e) Quantification of the relative expression levels of sqt, boz, and mkp3 in ich mutants and MO1-ccr7-injected ich mutants by qRT-PCR; * p<0.05, Student′s t test. (B) The effect of thapsigargin on the expression of mkp3 and sqt in WT and ich embryos at sphere stage, 4 hpf. (a–d) mkp3: b, n = 9/9; d, n = 13/13. (e–h) sqt: f, n = 6/6; h, n = 8/8. Animal views.

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Acknowledgments

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