ZFIN Image: Lindeman et al., 2012, Fig. 5

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Figure Caption

Fig. 5

C-Terminal Domains of Lrmp Facilitate Subcellular Targeting (A) Diagram of EFGP-fusion constructs. (B) Fusion construct RNAs encoding EGFP::LrmpCC-TM-L (top row), EGFP::LrmpTM-L (second row), and EGFP::LrmpCC construct (bottom two rows) were injected into one-cell WT embryos. Embryos expressing EGFP were fixed and processed for DAPI and anti-γ-tubulin immunostaining between 2.5 and 3.5 hpf. White boxes indicate fields shown at higher magnification (right). Scale bar represents 20 μm in all lower magnification panels. (C) Summary of results from EGFP::Lrmp C-terminal protein expression. Low viability (far right column) manifested as cell division defects and failure to undergo gastrulation, which led to embryo lysis. See also Figure S5.

Acknowledgments

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