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Fig. S5 Muscle pioneers and adaxial cells are present after 5 μM cyclopamine treatment. (A, B) Labeling with 4D9 antibody (anti-engrailed) showed that muscle pioneers are not missing after pharmacological inhibition of the Hedgehog pathway (arrows). (C–F) Also adaxial cells are still present, as shown by the expression of the markers myod (C, D) and smyhc1 (E, F). (A, B) Lateral views, dorsal is up. (C–F) Dorsal views, anterior is left.
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