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Fig. S2 ccdc80-l1 morpholino is capable to inhibit the expression of the fluorescent protein GFP. This assay was performed in order to verify the in vivo efficiency of ccdc80-l1-MO. (A, B) In the 70% of embryos injected with the ccdc80-l1-GFP sensor plasmid, the presence of fluorescent GFP signal was detected (N = 20). (C, D) When the plasmid was injected together with the morpholino, the transcription of GFP protein was inhibited and the percentage of fluorescent embryos decreased to 51% (N = 93). In A and C embryos are visualized under normal light, in B and D under fluorescent light.
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