ZFIN Image: Shen et al., 2012, Fig. 4

Image

Figure Caption

Fig. 4

Morpholinos reduced the levels of normal transcripts of mif-like, iclp1, and iclp2. (A) RT-PCR of mif-like transcripts from uninjected, control MO injected, mif morpholinos injected and mif morpholinos plus capped RNAs injected 50-hpf embryos. Predicted size of normal transcript is 512 bp; predicted transcript blocked by mif-like MO = 336 bp. (B) RT-PCR of iclp1 and iclp2 transcripts from control MO-injected, iclp morpholinos injected and iclp morpholinos plus capped RNAs injected 50-hpf embryos. Predicted size of normal iclp1 product = 523 bp, transcript lacking exon 2 = 353 bp. Predicted size of normal iclp2 product = 525 bp, transcript lacking exon 2 = 364 bp.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 363(1), Shen, Y.C., Thompson, D.L., Kuah, M.K., Wong, K.L., Wu, K.L., Linn, S.A., Jewett, E.M., Shu-Chien, A.C., and Barald, K.F., The cytokine macrophage migration inhibitory factor (MIF) acts as a neurotrophin in the developing inner ear of the zebrafish, Danio rerio, 84-94, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.