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Fig. 7
tcof1 loss-of-function adversely affects zebrafish craniofacial cartilage development.
Ventral (A–D), and dorsal views (E–F) (anterior to the left) of control (A, C, and E), and in6:ex7-MO+ex7:in7-MO-treated (B, D, and F) 4 dpf larvae stained with Alcian blue. Skeletal staining of control and morphants reveals hypoplasia of numerous craniofacial cartilages. Cranioskeletal hypoplasia is evident in the frontal, premaxillary, and maxillary elements. Abbreviations: cb1–5, ceratobranchial arches 1–5; ch, ceratohyal; ep, ethmoid plate; m, Meckel′s cartilage; pc, polar cartilage; pq, palatoquadrate; tr, trabecula.
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