ZFIN Image: Wong et al., 2012, Fig. 4

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Figure Caption

Fig. 4

Hedgehog signaling is required during 10–16.5 hpf period for endocardial differentiation based on NFATc1 expression analysis at 26 hpf. Flat-mounted embryos, dorsal view, anterior is to the top. (A) Cyclopamine addition at different stages of development shows that Hh signaling is required at least until approximately 16.5 hpf for endocardial NFATc1 and kdrl expression (arrows) while treatment after 19 hpf has little effect on the endocardium formation. Left panels, control embryos incubated in DMSO. Note the absence of endocardial tube in the embryos treated starting at 4 and 14 hpf. (B) Cyclopamine removal at different stages of development shows that Hh signaling is required from 10 hpf for endocardial NFATc1 and kdrl expression (arrows). Numbers indicate the treatment interval. (C,D) Percentage of embryos that display significant NFATc1 (blue bars) and kdrl (red bars) reduction (more than 50% of expression area is affected) at different stages of cyclopamine addition (C) and removal (D).

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 361(2), Wong, K.S., Rehn, K., Palencia-Desai, S., Kohli, V., Hunter, W., Uhl, J.D., Rost, M.S., and Sumanas, S., Hedgehog signaling is required for differentiation of endocardial progenitors in zebrafish, 377-91, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.