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Fig. S11 rbm24a and rbm24b splice blocked morphants display cardiac defects. Injection of 7.5 ng of rbm24a splice blocking morpholino results in a substantial reduction of full-length transcript (A). Injection of 9 ng of rbm24b splice blocking morpholino results in aberrant splicing of the transcript. There is a reduction of full-length transcript 250 bp fragment and appearance of trace amounts of the shortened frameshift fragment 164 bp and shortened in-frame transcript 109 bp (B). RT-PCR measurement of transcript levels show both rbm24a (42.80% +/- 3.35, P < 6.5 × 10-4) and rbm24b (40.27 +/- 3.19, P < 4.4 × 10-5) morphants have significant reduction of transcript levels compared to uninjected controls (C) Error bars are standard deviation, *** P < 0.001.