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Fig. 1 Fin amputation induces RA synthesis in the stump tissue. (A) qPCR determination of aldh1a2, rdh10b and rarga transcript levels at 6 hpa relative to uncut (0 hpa) fins. Error bars, s.e.m. *, P<0.01. (B,C) In situ hybridization on longitudinal section (B) and whole fins (C) demonstrates aldh1a2 expression in the stump mesenchyme. Note the absence of aldh1a2 transcripts in the most distal mesenchyme at 18 hpa. sm, stump mesenchyme; r, hemiray; we, wound epidermis. Dashed lines indicate amputation plane. Scale bars: 100 μm in B; 200 μm in C.
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