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Fig. S1 Similar Myc Levels and bcl-2 Suppression of Myc-induced Apoptosis in Premalignant Thymocytes of Myc;Cre and Myc;Cre;bcl-2 Transgenic Fish. (A) Western blot analyses reveal no significant differences in EGFP-mMyc protein levels in premaligant T-cells of Myc;Cre (n=4) versus Myc;Cre;bcl-2 (n=4) transgenic fish (P=0.67), despite variations of EGFP-mMyc levels among the individual fish and the striking levels of EGFP-zbcl-2 protein. Actin levels serve as a loading control. (B) FACS analysis of Annexin V-positive cells was performed on GFP-expressing control thymocytes of rag2-GFP (GFP) transgenic fish or premaligant thymocytes fromMyc;Cre and Myc;Cre;bcl-2 transgenic fish, respectively. Percentages of AnnexinV-positive cells for GFP vs. Myc;Cre; mean± SD: 8.72 ± 2.72 vs. 91.2 ± 15.77; Myc;Cre vs. Myc;Cre;bcl-2; mean± SD: 91.2 ± 15.77 vs. 49.13 ± 23.27; n=3 fish per group.
Reprinted from Cancer Cell, 18(4), Feng, H., Stachura, D.L., White, R.M., Gutierrez, A., Zhang, L., Sanda, T., Jette, C.A., Testa, J.R., Neuberg, D.S., Langenau, D.M., Kutok, J.L., Zon, L.I., Traver, D., Fleming, M.D., Kanki, J.P., and Look, A.T., T-lymphoblastic lymphoma cells express high levels of BCL2, S1P1, and ICAM1, leading to a blockade of tumor cell intravasation, 353-366, Copyright (2010) with permission from Elsevier. Full text @ Cancer Cell