ZFIN Image: Zuniga et al., 2011, Fig. 6

Image

Figure Caption

Fig. 6 Edn1 and Jag1b negatively regulate grem2 expression. (A-F) Confocal sections of in situ hybridization for grem2 (red) and dlx2a (green) in controls (A, n=62), jag1b^b1105 mutant (B, n=9), edn1^-/- mutant (C, n=5), UAS:Edn1 (D, n=17), hsp70I:dnBmpr1a-GFP (E, n=32) and UAS:Bmp4 (F, n=23) embryos at 36 hpf. In UAS:Edn1 embryos, grem2 expression was seen throughout the mandibular and hyoid arches, except for the ventral-most domain (white arrow). In hsp70I:dnBmpr1a-GFP embryos, grem2 expression shifted to ventral regions (yellow arrow) and was reduced dorsally (white arrowhead). Dorsal (D), intermediate (I) and ventral (V) domains of the mandibular (1) and hyoid (2) arches are labeled. Scale bar: 50 μm. (G,H) BRE:GFP expression increased throughout the dorsal and intermediate arch domains of edn1^-/- mutants (n=4) compared with wild-type siblings (n=16).

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development