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Fig. 5

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ZDB-IMAGE-111026-5
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Figures for Smith et al., 2011
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Fig. 5

Bmp via Bmpr1a regulates lefty1 expression in the midline.

(A) In situ hybridisation for lefty1 at 15-somites on embryos from an incross of bmpr1aa+/-;bmpr1ab+/- double carrier fish. Embryos were analysed for lefty1 expression and classified as robust (blue boxed panel) or reduced (red boxed panel) expression after which the embryos were genotyped. Quantification of the results is shown in the stacked area graph (blue, robust lefty1; red reduced lefty1). (B) In situ hybridisation for lefty1 at 10-somite stage. Embryos shown are Tg(hsp70l:bmp2b) embryos either heat-shocked at 10 hpf to induce bmp2b expression (left panel) or without heat-shock (middle panel) and Tg(hsp70l:nog3) embryos heat-shocked at 10 hpf to inhibit Bmp signalling (right panel). Lateral view of 10-somite stage embryos with dorsal to the right and anterior up.

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Acknowledgments
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