|
Fig. S3 Expression of growth factors is unchanged in LA1908 mutant embryos. Expression of bmp2b (A–D), bmp4 (E–H), and vegfaa (I–L) is indistinguishable between stage-matched wild type (A, C, E, G, I, K) and mutant (B, D, F, H, J, L) embryos. Furthermore, expression of vegfaa, the pro-angiogenic signal for ISV angiogenesis, persists in the ventral somites through the time-point after which apoptosis has resolved in LA1908 mutant embryos (K, L), whereas putative CVP pro-angiogenic signals in the ventral tail, bmp2b and bmp4, are absent from the ventral tail after one day of development (C, D, G, H), possibly explaining the differential ability of CVP versus ISV angiogenesis to recover after initial delay.