Fig. s4

Fig. s4

Spatial distribution of dorsal- and ventral-specific genes appears normal in maternally mutant mis embryos. Wild-type (A,C,E,G,I) and mis mutant (B,D,F,H,J) embryos exhibit normally restricted dorsal expression of nieuwkoid (A,B), goosecoid (C,D), foxb1.2 (E,F), and chordin (G,H), as well as normally restricted ventral expression of even skipped (I,J). (A–D) are embryos at 5 h.p.f., (E–F) at 7.5 h.p.f., and (G-J) at 8 h.p.f. All panels are animal pole views with dorsal to the right. Even though goosecoid expression is normally restricted, its levels of expression often appear reduced compared to wild-type. We have not further explored this effect, although it may be related to a requirement of mis function in the activation of additional nodal target genes such as goosecoid (Chen and Schier, 2001).