Fig. S3 Levels of BMP activity upon induction of the various transgenes. Confocal projections from control (A,A′,E,E′), Tg(hs:dnBMPr1a-CFP) transgenic (B,B′), Tg(hsp70:noggin3) transgenic (C,C′), Tg(hs:dnBMPr1a-CFP);Tg(hsp70:noggin3) double-transgenic (D,D′); Tg(hs:BMP2b) transgenic (F,F′) and Tg(hs:Gal4); Tg(UAS:caBMPr1a) double-transgenic (G-G′) embryos double-labeled with an α-PSmad 1/5/8 antibody (grey) and a Tg(HuC:GFP) transgene at 20 (A-D′) and 24 hours (E-G′). The Tg(HuC:GFP) transgene helps delineating the posterior limit of the pineal gland. The pineal area is indicated with a bracket. Transgenic and control embryos were heat shocked at 16 (A-D′) or 21 hours (E-G′). Anterior is upwards. Scale bars: 16 µm.
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