Fig. 1

Fig. 1 Identification and characterization of git2 genes in zebrafish. (A) Phylogenetic analysis of zebrafish git2 family genes. Dendogram of zebrafish git2a on chromosome 5 and git2b on chromosome 10 and related orthologs from other species. (B) In situ hybridization of git2a mRNA expression in the zebrafish embryo. git2a expression was ubiquitously detected at the 4-cell, epiboly, tailbud and 14-somite (14SS) and 24 hpf stages. (C) Western blotting of zebrafish Git2 protein at dome, 50%, 75%, 90% epiboly and 6-somite (6SS) stages, α-tubulin and paxillin were used as loading controls. (D) Immunohistochemistry of Git2 (red) at the 75% epiboly stage. Embryos were co-stained with phalloidin to detect F-actin (green). Images show surface EVL cells and deep cells (30 μm below the surface). Scale bar, 50 μm. Fluorescent intensity profiles show relative F-actin (green) and Git2 (red) levels in EVL cells at the blastoderm margin (1) and deep cells (2). (E) Immunohistochemistry of Git2 (red) at the 24 hpf. Embryos were co-stained with phalloidin to detect F-actin (green). Scale bar, 50 μm. Zebrafish embryo drawings are adapted from Kimmel et al., 1995.