ZFIN Image: Schneider et al., 2010, Fig. 6

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Figure Caption

Fig. 6 Nkd1 promotes Dvl degradation and impacts CE movements. (A) Westerm blot of zebrafish embryos injected with myc-tagged Dvl2 only, or coinjected with either nkd1 RNA or egfp RNA. The first lane is uninjected embryos, anti-β-actin was used as loading control. Embryos were frozen at 80% epiboly and equal amounts of cell lysates were used for Western blot analysis. (B) Bright field and (C) fluorescence images of embryos at 90% epiboly, injected with Dvl-GFP only (left) or coinjected with nkd1 RNA (right). (D) Dvl-GFP (green) and Topro3 nuclear staining (red) denotes Dvl localization in wt and (E) Dvl-GFP coinjected with nkd1 RNA. (F) krox20 and (G) myoD markers in uninjected and (H) krox20 and (I) myoD markers nkd1 RNA-injected zebrafish embryos. 43% of nkd1 injected embryos show CE defects, n = 143. Dorsal view, anterior to the left.

Acknowledgments

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Reprinted from Developmental Biology, 348(1), Schneider, I., Schneider, P.N., Derry, S.W., Lin, S., Barton, L.J., Westfall, T., and Slusarski, D.C., Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning, 22-33, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.