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Fig. S2 Loss of miR-145 leads to smooth muscle marker expression changes. (A) RT-PCR shows increased expression of nm-mhc-b and smoothelin in miR-145 morphants at 54, 72, and 96 hpf in comparison with the housekeeping gene EF1α. (B) qPCR indicates significantly increased expression of gata6 and sm22α-b but similar level of αsma in 48 hpf embryos treated with pre-miR-145 morpholino. (C) qPCR shows the increased expression of nm-mhc-b in miR-145 MO or pre-miR-145 morpholino treated embryos at 48 hpf. Bars, mean ± SEM. *, P < 0.05.
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