ZFIN Image: Yin et al., 2010, Fig. S2

Image

Figure Caption

Fig. S2 The LPM in the gut-looping region of han^c99 mutants does not exhibit impaired epithelial polarity or altered Fibronectin deposition
(A-B) In wild-type and han^c99-/- embryos, ZO-1 (red) is localized to the apical side of the LPM epithelium. Dashed lines outline the LPM.
(C-D) Expression of fibronectin1/fn1 gene within the gut-looping region in wild-type and han^{c99- /-} embryos. Dashed lines outline the notochord (NC) and the LPM.
(E-F) Deposition of Fn protein along the LPM/gut boundary appeared to be comparable between wild-type (E) and han^c99-/- (F) embryos at 30 hpf.
(G-H) Deposition of Fn also appeared to be comparable between wild-type embryos treated with DMSO (G) and embryos treated with 10 μM of the MMP inhibitor GM6001 (H) between 24 and 30 hpf. All images show transverse sections, dorsal to the top. In (E-H), asterisks mark Fn deposition along the LPM/gut boundary. L, left; R, right. Scale bars, 40 μm.

Acknowledgments

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Reprinted from Developmental Cell, 18(6), Yin, C., Kikuchi, K., Hochgreb, T., Poss, K.D., and Stainier, D.Y., Hand2 Regulates Extracellular Matrix Remodeling Essential for Gut-Looping Morphogenesis in Zebrafish, 973-984, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell